Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a
rabbit affinity purified polyclonal antibody raised against
amino acid 163-175 of murine caspase 3 (9). This neo epitope
is present on the p18 subunit of cleaved caspase 3 (9).
Cells undergoing apoptosis are fixed and permeablized prior
to the addition of the antibody. A secondary FITC labeled
goat anti rabbit antibody is used to visualize the bound
rabbit anti caspase 3 polyclonal.