Cell Technology’s Nonmammalian-Based Assay Diluent for serum and
plasma samples has been specially formulated for antigen-down ELISA
formats. EAD contains a non-mammalian protein base designed to
minimize nonspecific binding of the sample matrix to "sticky"
adsorbed protein antigens. Specially selected detergents have also
been added to enhance the specific signal without denaturing
immobilized proteins, helpful when evaluating human, porcine, and
bovine serum samples. EAD300 also contains proprietary additives
designed to inhibit complement and thrombin activity present in
serum and plasma samples. Nonmammalian-Based Assay Diluent does not
contain any immunoglobulin-based components that could interfere
with anti-IgG signal-generating formats.