Effects of Three-Month Feeding High Fat Diets with Different Fatty Acid Composition on Myocardial Proteome in Mice

Effects of Three-Month Feeding High Fat Diets with Different Fatty Acid Composition on Myocardial Proteome in Mice

Westernized diet is characterized by a high content of saturated fatty acids (SFA) and a low level of omega-<em>3</em> polyunsaturated fatty acids (PUFA), often accompanied by an imbalance in the omega-6/omega-<em>3</em> PUFA ratio. Since increased intake of SFA and n-6 PUFA is considered as a cardiovascular disease risk factor, this study was conducted to determine whether a three-month dietary supplementation of high-fat diets (HFDs) with saturated fatty acids and a significant proportion of various n-6 and n-<em>3</em> PUFA ratios would affect the architecture and <em>protein</em> expression patterns of the murine heart.

Therefore, three HFD (<i>n</i> = 6) feeding groups: rich in SFA, dominated by PUFA with the n-6/n-<em>3</em>-14:1, and n-6/n-<em>3</em>-5:1, ratios were compared to animals fed standard mouse chow. For this purpose, we performed two-dimensional electrophoresis with MALDI-ToF mass spectrometry-based identification of differentially expressed cardiac <em>proteins</em>, and a histological examination of cardiac morphology. The results indicated that mice fed with all HFDs developed signs of hypertrophy and cardiac fibrosis. Animals fed SFA-rich HFD manifested the most severe cardiac hypertrophy and fibrosis lesions, whereas less pronounced changes were observed in the group of animals that ingested the highest amount of omega-<em>3</em> FA.

In general, all HFDs, regardless of FA composition, evoked a comparable pattern of cardiac <em>protein</em> changes and affected the following biological processes: lipid metabolism and FA β-oxidation, glycolysis, <em>TCA</em> cycle, respiratory chain, myocardium contractility, oxidative stress and PUFA eicosanoid metabolism. However, it should be noted that three <em>proteins</em>, namely IDH<em>3</em>A, LDHB, and AK1, were affected differently by various FA contents. High expression of these myocardial <em>proteins</em> found in the group of animals fed a HFD with the highest n-<em>3</em> PUFA content could be closely related to the observed development of hypertrophy.

LC/MS-Based Global Metabolomic Identification of Serum Biomarkers Differentiating Hepatocellular Carcinoma from Chronic Hepatitis B and Liver Cirrhosis

 

Chronic hepatitis B virus (CHB) infection is one of the primary risk factors associated with the development of hepatocellular carcinoma (HCC). Despite having been extensively studied, diagnosing early-stage HCC remains challenging, and diagnosed patients have a poor (<em>3</em>-5%) survival rate. Identifying new approaches to detect changes in the serum metabolic profiles of patients with CHB and liver cirrhosis (LC) may provide a valuable approach to better detect HCC at an early stage when it is still amenable to treatment, thereby improving patient prognosis and survival. In the present study, we, therefore, employed a liquid chromatography-mass spectrometry (LC-MS)-based approach to evaluate the serum metabolic profiles of <em>3</em>0 CHB patients, 29 LC patients, and <em>3</em>0 HCC patients. We then employed appropriate statistical methods to identify those metabolites that were best able to distinguish HCC cases from LC and CHB controls.

Effects of Three-Month Feeding High Fat Diets with Different Fatty Acid Composition on Myocardial Proteome in Mice

A mass-based database was then used to putatively identify these metabolites. We then confirmed the identities of a subset of these metabolites through comparisons with the MS/MS fragmentation patterns and retention times of reference standards. The serum samples were then reanalyzed to quantify the levels of these selected metabolites and of other metabolites that have previously been identified as potential HCC biomarkers. Through this approach, we observed clear differences in the metabolite profiles of the CHB, LC, and HCC patient groups in both positive- and negative-ion modes.

Reduced Fragmentation of IGFBP-2 and IGFBP-<em>3</em> as a Potential Mechanism for Decreased Ratio of IGF-II to IGFBPs in Cerebrospinal Fluid in Response to Repeated Intrathecal Administration of Triamcinolone Acetonide in Patients With Multiple Sclerosis

 

Multiple sclerosis (MS) is a chronic autoimmune disease of the brain and spinal cord causing a wide range of symptoms such as impaired walking capability, spasticity, fatigue, and pain. The insulin-like growth factor (IGF) system has regulatory functions for the induction of inflammatory pathways in experimental encephalomyelitis. We have therefore assessed expression and regulation of the IGF system on the level of IGFs and IGFBPs in serum and cerebrospinal fluid (CSF) in the course of four repeated triamcinolone acetonide (<em>TCA</em>) administrations in two female and four male MS patients.

Sample series of 20 treatment cycles were analyzed. IGF-I and IGF-II were quantified by ELISAs, and IGFBPs were analyzed by quantitative Western ligand (qWLB) and Western immunoblotting (WIB) in order to differentiate intact and fragmented IGFBPs. The ratios of fragmented to intact IGFBP-2 and -<em>3</em> were calculated in serum and CSF. Finally, the ratios of IGF-I and IGF-II to the total IGF-binding activity, quantified by qWLB, were determined as an indicator of IGF-related bioactivity.

After the fourth <em>TCA</em> administration, the average level of IGF-I was increased in serum (p < 0.001). The increase of IGF-I concentrations in serum resulted in an increased ratio of IGF-I to IGFBPs in the circulation. By contrast in CSF, fragmentation of IGFBP-2 and IGFBP-<em>3</em> and the ratio of IGF-II to intact IGFBPs were decreased at the fourth <em>TCA</em> administration (p < 0.01).

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

LEC Chemokine(LEC66) Antibody

BNC040066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF405S conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC040066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF405S conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC040067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF405S conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC040067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF405S conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC470066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF647 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC470066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF647 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC470067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF647 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC470067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF647 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC550066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF555 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC550066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF555 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC550067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF555 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC550067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF555 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCB0066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), Biotin conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCB0066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), Biotin conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCB0067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), Biotin conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCB0067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), Biotin conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC810066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF680R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC810066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF680R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC810067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF680R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC810067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF680R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC700066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF770 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC700066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF770 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC700067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF770 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC700067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF770 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC880066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF488A conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC880066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF488A conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC880067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF488A conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC880067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF488A conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC940066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF594 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC940066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF594 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC940067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF594 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC940067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF594 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC680066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF568 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC680066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF568 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC680067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF568 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC680067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF568 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCA0066-250 250uL
EUR 383
Description: Primary antibody against LEC Chemokine(LEC66), APC conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCA0067-250 250uL
EUR 383
Description: Primary antibody against LEC Chemokine(LEC67), APC conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCAP0066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCAP0066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCAP0067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCAP0067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNUM0066-50 50uL
EUR 395
Description: Primary antibody against LEC Chemokine(LEC66), 1mg/mL

LEC Chemokine(LEC67) Antibody

BNUM0067-50 50uL
EUR 395
Description: Primary antibody against LEC Chemokine(LEC67), 1mg/mL

LEC Chemokine(LEC66) Antibody

BNCP0066-250 250uL
EUR 383
Description: Primary antibody against LEC Chemokine(LEC66), PerCP conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCP0067-250 250uL
EUR 383
Description: Primary antibody against LEC Chemokine(LEC67), PerCP conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC050066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF405M conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC050066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF405M conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC050067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF405M conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC050067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF405M conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC400066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF640R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC400066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF640R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC400067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF640R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC400067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF640R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCR0066-250 250uL
EUR 383
Description: Primary antibody against LEC Chemokine(LEC66), RPE conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCR0067-250 250uL
EUR 383
Description: Primary antibody against LEC Chemokine(LEC67), RPE conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNUB0066-100 100uL
EUR 209
Description: Primary antibody against LEC Chemokine(LEC66), Concentration: 0.2mg/mL

LEC Chemokine(LEC66) Antibody

BNUB0066-500 500uL
EUR 458
Description: Primary antibody against LEC Chemokine(LEC66), Concentration: 0.2mg/mL

LEC Chemokine(LEC67) Antibody

BNUB0067-100 100uL
EUR 209
Description: Primary antibody against LEC Chemokine(LEC67), Concentration: 0.2mg/mL

LEC Chemokine(LEC67) Antibody

BNUB0067-500 500uL
EUR 458
Description: Primary antibody against LEC Chemokine(LEC67), Concentration: 0.2mg/mL

LEC Chemokine(LEC66) Antibody

BNC430066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF543 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC430066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF543 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC430067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF543 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC430067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF543 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCH0066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNCH0066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCH0067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNCH0067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC610066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF660R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC610066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF660R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC610067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF660R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC610067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF660R conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC800066-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC66), CF680 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC66) Antibody

BNC800066-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC66), CF680 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC800067-100 100uL
EUR 199
Description: Primary antibody against LEC Chemokine(LEC67), CF680 conjugate, Concentration: 0.1mg/mL

LEC Chemokine(LEC67) Antibody

BNC800067-500 500uL
EUR 544
Description: Primary antibody against LEC Chemokine(LEC67), CF680 conjugate, Concentration: 0.1mg/mL

Recombinant Human LEC/NCC-4 (CCL16)

7-01792 5µg Ask for price

Recombinant Human LEC/NCC-4 (CCL16)

7-01793 20µg Ask for price

Recombinant Human LEC/NCC-4 (CCL16)

7-01794 1mg Ask for price

LEC / NCC-4 (CCL16) Protein

20-abx260667
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

HCC-4, LEC, NCC-4, CCL16, human

RC315-27 5ug
EUR 104.38

CCL16 LEC/NCC-4 Human Recombinant Protein

PROTO15467 Regular: 20ug
EUR 317
Description: CCL16 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 97 amino acids and having a molecular mass of 11.2 kDa. ;The CCL16 is purified by proprietary chromatographic techniques.

Recombinant Caenorhabditis Elegans lec-7 Protein (aa 1-179)

VAng-Ly3505-1mgEcoli 1 mg (E. coli)
EUR 3335
Description: Caenorhabditis Elegans probable galaptin lec-7 (lec-7), recombination protein.

Recombinant Caenorhabditis Elegans lec-7 Protein (aa 1-179)

VAng-Ly3505-500gEcoli 500 µg (E. coli)
EUR 2388
Description: Caenorhabditis Elegans probable galaptin lec-7 (lec-7), recombination protein.

Recombinant Caenorhabditis Elegans lec-7 Protein (aa 1-179)

VAng-Ly3505-50gEcoli 50 µg (E. coli)
EUR 1645
Description: Caenorhabditis Elegans probable galaptin lec-7 (lec-7), recombination protein.

MBP/MBL ELISA Kit| Rat Mannma Binding Protein/Mannan Binding Lec

EF017876 96 Tests
EUR 689

Monoclonal HCC-4 / Liver Expressed Chemokine (LEC) Antibody - With BSA and Azide, Clone: LEC66

AMM00148G 0.05mg
EUR 396
Description: A Monoclonal antibody against Human HCC-4 / Liver Expressed Chemokine (LEC) - With BSA and Azide. The antibodies are raised in Mouse and are from clone LEC66. This antibody is applicable in E

Monoclonal HCC-4 / Liver Expressed Chemokine (LEC) Antibody - With BSA and Azide, Clone: LEC67

AMM00149G 0.05mg
EUR 396
Description: A Monoclonal antibody against Human HCC-4 / Liver Expressed Chemokine (LEC) - With BSA and Azide. The antibodies are raised in Mouse and are from clone LEC67. This antibody is applicable in E

Monoclonal HCC-4 / Liver Expressed Chemokine (LEC) Antibody - Without BSA and Azide, Clone: LEC66

AMM00393G 0.1mg
EUR 484
Description: A Monoclonal antibody against Human HCC-4 / Liver Expressed Chemokine (LEC) - Without BSA and Azide. The antibodies are raised in Mouse and are from clone LEC66. This antibody is applicable in E

Furthermore, reduced fragmentation of IGFBP-<em>3</em> in CSF was accompanied by increased concentrations of intact IGFBP-<em>3</em> (p < 0.001). We conclude that reduced fragmentation of IGFBPs and concomitant reduction of IGF-II to IGFBP ratios indicate regulation of bioactivity of IGF-II in CSF during repeated intrathecal <em>TCA</em> administration in MS patients.