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Fluoro EPO™
Fluorescent Eosinophil Peroxidase Detection Kit
 
Key Benefits:
  • Can monitor multiple time points to follow kinetics.
  • One-step, no wash assay.
  • Adaptable for High Throughput format
  • Highly Sensitive
  • Applications - Fluorescence (Plate Reader) or Absorbance
Assay Principle
Eosinophil peroxidase (EPO) is the most abundant enzyme found in eosinophils. It is the major cytotoxic agent released by activated eosinophils and uses hydrogen peroxide to generate reactive oxidants from halides and pseudo halide thiocyanate [1,2]. Eosinophils peroxidase has been shown to have antimycobacterial activity [3], however it is also implicated in tissue damage that occurs in asthma and other diseases [4,5].  Currently, the function of eosinophil involvement in the immune response is being redefined. Once considered a cell involved in host protection of parasitic infection, eosinophils multiple functions as leukocytes involved in the initiation and propagation of diverse inflammatory responses are being investigated. Eosinophils are further involved as modulators of innate and adaptive immunity [6].
H2O2 + Detection reagent (non-fluorescent)+ EPO
                                             ---------> fluorescent analog 
                                     Excitation:530-571nm; Emission: 590-600nm
Applications
  • Detection of EPO activity in isolated eosinophils.
  • Functional studies of eosinophil degranulation.
  • In-vitro eosinophil chemotaxis studies.
Kit contents (for 100 assays)
  1. 5X Reaction Buffer: 60ml   ..........................................Part 3011
  2. Detection reagent: One vial for 100 assays....................Part 4007
  3. Hydrogen Peroxide: 1000µL of a stabilized 3% solution...Part 3012
  4. Eosinophil Peroxidase: 100µL in 1 vial at 10 units/ml......Part 6016
The following kits are available:
Catalog 
 
Size
 
Price (Eur)
FLEPO 100-2
 
100 Tests €395