Fast phosphine-activated control of protein function using unnatural lysine analogues.

Effective, general methods for conditionally activating proteins in their native biological environments are highly useful for biological studies. Since phosphines and azides are not found in pro- and eukaryotic cells, the Staudinger reduction can function as an excellent small molecule-controlled switch for protein activation.

This methodology involves site-specifically incorporating azidobenyl-lysine analogues into proteins in live cells. When placed at a crucial position, these unnatural side chains block protein function until a phosphine trigger is added.

We discuss methods for expressing caged proteins in bacterial and mammalian cells in high yields, and activating the proteins with an optimized phosphine trigger. We also discuss important considerations for safe and effective synthesis of these molecules. This methodology was used to translocate proteins to the nucleus and to turn-on a protein post-translational modification (SUMOylation) in living cells.

KN-62

A8180-5 5 mg
EUR 131
Description: KN-62, 1-[N,O-bis-(5-isoquinolinesulphonyl)-N-methyl-L-tyrosy]-4-phenylpiperazine is a highly selective inhibitor of the calcium dependent calmodulin protein kinase II (CAMKII), which binds to the calmodulin binding site of CAMKII.

KN-62

A8180-5.1 10 mM (in 1mL DMSO)
EUR 195
Description: KN-62, 1-[N,O-bis-(5-isoquinolinesulphonyl)-N-methyl-L-tyrosy]-4-phenylpiperazine is a highly selective inhibitor of the calcium dependent calmodulin protein kinase II (CAMKII), which binds to the calmodulin binding site of CAMKII.

KN-62

A8180-S Evaluation Sample
EUR 81
Description: KN-62, 1-[N,O-bis-(5-isoquinolinesulphonyl)-N-methyl-L-tyrosy]-4-phenylpiperazine is a highly selective inhibitor of the calcium dependent calmodulin protein kinase II (CAMKII), which binds to the calmodulin binding site of CAMKII.

KN-62

2495-1
EUR 185

KN-62

2495-5
EUR 588

UACC-62 cells

C0020003 One Frozen vial
EUR 485

pGreenII- 62- SK

PVT11188 2 ug
EUR 370

anti-CGI 62

YF-PA18846 50 ul
EUR 363
Description: Mouse polyclonal to Anti-CGI 62

anti-CGI 62

YF-PA18847 50 ug
EUR 363
Description: Mouse polyclonal to Anti-CGI 62

KBA.62 (Melanoma Associated Antigen); Clone KBA.62 (Concentrate)

RA0416-C.1 0.1 ml
EUR 125

KBA.62 (Melanoma Associated Antigen); Clone KBA.62 (Concentrate)

RA0416-C.5 0.5 ml
EUR 300

Anti-KBA.62 Antibody Clone KBA.62, Unconjugated-100ug

MSM1-895-P1 100ug
EUR 428

anti-Rpn3 (yeast) antibody, rabbit serum

62-201 100uL
EUR 453
Description: The anti-Rpn3 (yeast) antibody, rabbit serum is available in Europe and for worldwide shipping via Gentaur.

anti-Rpn5 (yeast) antibody, rabbit purified antibody

62-203 100uL
EUR 453
Description: The anti-Rpn5 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Rpn7 (yeast) antibody, rabbit purified antibody

62-205 100uL
EUR 453
Description: The anti-Rpn7 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Rpn9 (yeast) antibody, rabbit purified antibody

62-207 100uL
EUR 479
Description: The anti-Rpn9 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Rpn12 (yeast) antibody, rabbit purified antibody

62-209 20uL
EUR 375
Description: The anti-Rpn12 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Nob1 (yeast) antibody, rabbit purified antibody

62-211 20uL
EUR 479
Description: The anti-Nob1 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Nas6 (yeast) antibody, rabbit purified antibody

62-213 100uL
EUR 479
Description: The anti-Nas6 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Tem1 (yeast) antibody, rabbit purified antibody

62-215 100uL
EUR 479
Description: The anti-Tem1 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

HBsAg antibody (Gold Colloid)

62-H05B 10 ml
EUR 457
Description: Mouse monoclonal HBsAg antibody (Gold Colloid)

hCG beta antibody (Gold Colloid)

62-H25C 10 ml
EUR 505
Description: Mouse monoclonal hCG beta antibody (Gold Colloid)

Luteinizing Hormone alpha antibody (Gold Colloid)

62-L26B 10 ml
EUR 527
Description: Mouse monoclonal Gold Colloid conjugated Luteinizing Hormone Alpha antibody

THC antibody (Gold Colloid)

62-T43A 10 ml
EUR 506
Description: Mouse monoclonal THC antibody conjugated to gold colloid

Recombinant Nucleoporin 62 (NUP62)

4-RPC257Ra01
  • EUR 530.08
  • EUR 245.00
  • EUR 1712.80
  • EUR 637.60
  • EUR 1175.20
  • EUR 418.00
  • EUR 4132.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Nucleoporin 62 expressed in: E.coli

HIST1H1C (Ab-62) Antibody

1-CSB-PA010378OA62ncrHU
  • EUR 332.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against HIST1H1C (Ab-62). Recognizes HIST1H1C (Ab-62) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:10-1:100

HIST1H1C (Ab-62) Antibody

1-CSB-PA010378OA62nsucHU
  • EUR 332.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against HIST1H1C (Ab-62). Recognizes HIST1H1C (Ab-62) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

HIST1H1C (Ab-62) Antibody

1-CSB-PA010378PA62nacHU
  • EUR 332.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against HIST1H1C (Ab-62). Recognizes HIST1H1C (Ab-62) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

HIST1H1E (Ab-62) Antibody

1-CSB-PA010380PA62nforHU
  • EUR 332.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against HIST1H1E (Ab-62). Recognizes HIST1H1E (Ab-62) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200

STMN1 (Ab-62) Antibody

CSB-PA082087-
EUR 335
Description: A polyclonal antibody against STMN1 (Ab-62). Recognizes STMN1 (Ab-62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200

STMN1 (Ab-62) Antibody

CSB-PA082087-100ul 100ul
EUR 316
Description: A polyclonal antibody against STMN1 (Ab-62). Recognizes STMN1 (Ab-62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200

BCL2L1 (Ab-62) Antibody

CSB-PA923588-
EUR 335
Description: A polyclonal antibody against BCL2L1 (Ab-62). Recognizes BCL2L1 (Ab-62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

BCL2L1 (Ab-62) Antibody

CSB-PA923588-100ul 100ul
EUR 316
Description: A polyclonal antibody against BCL2L1 (Ab-62). Recognizes BCL2L1 (Ab-62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

stathmin1 (Ab-62) Antibody

21518-100ul 100ul
EUR 252

stathmin1 (Ab-62) Antibody

21518-50ul 50ul
EUR 187

ATF2 (pS44 / 62) Antibody

20-abx119178
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Nucleoporin 62 (NUP62) Antibody

20-abx001943
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Nucleoporin 62 (NUP62) Antibody

20-abx142112
  • EUR 370.00
  • EUR 606.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 50 ul

Nucleoporin 62 (NUP62) Antibody

abx033433-400ul 400 ul
EUR 523

Nucleoporin 62 (NUP62) Antibody

abx033433-80l 80 µl
EUR 286

Nucleoporin 62 (NUP62) Antibody

abx433060-200ul 200 ul
EUR 286

Nucleopurin 62 kDa Protein

20-abx262076
  • EUR 4838.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Nucleoporin 62 (NUP62) Antibody

abx235927-100ug 100 ug
EUR 481

anti-stathmin1 (Ab-62)

LF-PA20689 100 ul
EUR 334
Description: Rabbit polyclonal to stathmin1

KBA 62/Melanoma Associated Antigen

MO47032 100 ul
EUR 349

Recombinant HPV-62 L2 Protein

VAng-Wyb3365-inquire inquire Ask for price
Description: Human papillomavirus type 62 Major capsid protein L1, recombinant protein.

BCL-XL (Ab-62) Antibody

21061-100ul 100ul
EUR 252

BCL-XL (Ab-62) Antibody

21061-50ul 50ul
EUR 187

Nucleoporin 62 kDa (NUP62) Antibody

20-abx128968
  • EUR 314.00
  • EUR 133.00
  • EUR 815.00
  • EUR 425.00
  • EUR 272.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Nucleoporin 62 kDa (NUP62) Antibody

20-abx114230
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Melanoma Marker(KBA.62) Antibody

BNC040895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF405S conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC040895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF405S conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC470895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF647 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC470895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF647 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNCAP0895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNCAP0895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNCB0895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), Biotin conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNCB0895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), Biotin conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC810895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF680R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC810895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF680R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC700895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF770 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC700895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF770 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC880895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF488A conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC880895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF488A conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC610895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF660R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC610895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF660R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC680895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF568 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC680895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF568 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNCA0895-250 250uL
EUR 383
Description: Primary antibody against Melanoma Marker(KBA.62), APC conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNUM0895-50 50uL
EUR 395
Description: Primary antibody against Melanoma Marker(KBA.62), 1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC050895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF405M conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC050895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF405M conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC400895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF640R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC400895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF640R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNCP0895-250 250uL
EUR 383
Description: Primary antibody against Melanoma Marker(KBA.62), PerCP conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNCR0895-250 250uL
EUR 383
Description: Primary antibody against Melanoma Marker(KBA.62), RPE conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNUB0895-100 100uL
EUR 209
Description: Primary antibody against Melanoma Marker(KBA.62), Concentration: 0.2mg/mL

Melanoma Marker(KBA.62) Antibody

BNUB0895-500 500uL
EUR 458
Description: Primary antibody against Melanoma Marker(KBA.62), Concentration: 0.2mg/mL

Nucleoporin 62 kDa (NUP62) Antibody

20-abx242160
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Nucleoporin 62 kDa (NUP62) Antibody

20-abx242161
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Nucleoporin 62 kDa (NUP62) Antibody

20-abx173878
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug

Melanoma Marker(KBA.62) Antibody

BNC430895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF543 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC430895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF543 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNCH0895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNCH0895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC550895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF555 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC550895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF555 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC940895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF594 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC940895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF594 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC800895-100 100uL
EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF680 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody

BNC800895-500 500uL
EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF680 conjugate, Concentration: 0.1mg/mL

stathmin1 (Ab-62) Conjugated Antibody

C21518 100ul
EUR 397

CART (62-76) (rat, human)

B5414-1 1 mg
EUR 383

CART (62-76) (human, rat)

H-5098.0001 1.0mg
EUR 576
Description: Sum Formula: C64H99N17O23S3; CAS# [210978-19-1] net

CART (62-76) (human, rat)

H-5098.0005 5.0mg
EUR 2207
Description: Sum Formula: C64H99N17O23S3; CAS# [210978-19-1] net

anti-BCL-XL (Ab-62)

LF-PA20050 100 ul
EUR 334
Description: Rabbit polyclonal to BCL-XL