Fast phosphine-activated control of protein function using unnatural lysine analogues.

Effective, general methods for conditionally activating proteins in their native biological environments are highly useful for biological studies. Since phosphines and azides are not found in pro- and eukaryotic cells, the Staudinger reduction can function as an excellent small molecule-controlled switch for protein activation.

This methodology involves site-specifically incorporating azidobenyl-lysine analogues into proteins in live cells. When placed at a crucial position, these unnatural side chains block protein function until a phosphine trigger is added.

We discuss methods for expressing caged proteins in bacterial and mammalian cells in high yields, and activating the proteins with an optimized phosphine trigger. We also discuss important considerations for safe and effective synthesis of these molecules. This methodology was used to translocate proteins to the nucleus and to turn-on a protein post-translational modification (SUMOylation) in living cells.

KN-62
A8180-5	ApexBio	5 mg	EUR 131
Description: KN-62, 1-[N,O-bis-(5-isoquinolinesulphonyl)-N-methyl-L-tyrosy]-4-phenylpiperazine is a highly selective inhibitor of the calcium dependent calmodulin protein kinase II (CAMKII), which binds to the calmodulin binding site of CAMKII.

KN-62
A8180-5.1	ApexBio	10 mM (in 1mL DMSO)	EUR 195
Description: KN-62, 1-[N,O-bis-(5-isoquinolinesulphonyl)-N-methyl-L-tyrosy]-4-phenylpiperazine is a highly selective inhibitor of the calcium dependent calmodulin protein kinase II (CAMKII), which binds to the calmodulin binding site of CAMKII.

KN-62
A8180-S	ApexBio	Evaluation Sample	EUR 81
Description: KN-62, 1-[N,O-bis-(5-isoquinolinesulphonyl)-N-methyl-L-tyrosy]-4-phenylpiperazine is a highly selective inhibitor of the calcium dependent calmodulin protein kinase II (CAMKII), which binds to the calmodulin binding site of CAMKII.

KN-62
2495-1	Biovision		EUR 185

KN-62
2495-5	Biovision		EUR 588

UACC-62 cells
C0020003	Addexbio	One Frozen vial	EUR 485

pGreenII- 62- SK
PVT11188	Lifescience Market	2 ug	EUR 370

anti-CGI 62
YF-PA18846	Abfrontier	50 ul	EUR 363
Description: Mouse polyclonal to Anti-CGI 62

anti-CGI 62
YF-PA18847	Abfrontier	50 ug	EUR 363
Description: Mouse polyclonal to Anti-CGI 62

KBA.62 (Melanoma Associated Antigen); Clone KBA.62 (Concentrate)
RA0416-C.1	ScyTek Laboratories	0.1 ml	EUR 125

KBA.62 (Melanoma Associated Antigen); Clone KBA.62 (Concentrate)
RA0416-C.5	ScyTek Laboratories	0.5 ml	EUR 300

Anti-KBA.62 Antibody Clone KBA.62, Unconjugated-100ug
MSM1-895-P1	EnQuireBio	100ug	EUR 428

anti-Rpn3 (yeast) antibody, rabbit serum
62-201	Sceti	100uL	EUR 453
Description: The anti-Rpn3 (yeast) antibody, rabbit serum is available in Europe and for worldwide shipping via Gentaur.

anti-Rpn5 (yeast) antibody, rabbit purified antibody
62-203	Sceti	100uL	EUR 453
Description: The anti-Rpn5 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Rpn7 (yeast) antibody, rabbit purified antibody
62-205	Sceti	100uL	EUR 453
Description: The anti-Rpn7 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Rpn9 (yeast) antibody, rabbit purified antibody
62-207	Sceti	100uL	EUR 479
Description: The anti-Rpn9 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Rpn12 (yeast) antibody, rabbit purified antibody
62-209	Sceti	20uL	EUR 375
Description: The anti-Rpn12 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Nob1 (yeast) antibody, rabbit purified antibody
62-211	Sceti	20uL	EUR 479
Description: The anti-Nob1 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Nas6 (yeast) antibody, rabbit purified antibody
62-213	Sceti	100uL	EUR 479
Description: The anti-Nas6 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

anti-Tem1 (yeast) antibody, rabbit purified antibody
62-215	Sceti	100uL	EUR 479
Description: The anti-Tem1 (yeast) antibody, rabbit purified antibody is available in Europe and for worldwide shipping via Gentaur.

HBsAg antibody (Gold Colloid)
62-H05B	Fitzgerald	10 ml	EUR 457
Description: Mouse monoclonal HBsAg antibody (Gold Colloid)

hCG beta antibody (Gold Colloid)
62-H25C	Fitzgerald	10 ml	EUR 505
Description: Mouse monoclonal hCG beta antibody (Gold Colloid)

Luteinizing Hormone alpha antibody (Gold Colloid)
62-L26B	Fitzgerald	10 ml	EUR 527
Description: Mouse monoclonal Gold Colloid conjugated Luteinizing Hormone Alpha antibody

THC antibody (Gold Colloid)
62-T43A	Fitzgerald	10 ml	EUR 506
Description: Mouse monoclonal THC antibody conjugated to gold colloid

Recombinant Nucleoporin 62 (NUP62)
4-RPC257Ra01	Cloud-Clone	EUR 530.08 EUR 245.00 EUR 1712.80 EUR 637.60 EUR 1175.20 EUR 418.00 EUR 4132.00	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Rat Nucleoporin 62 expressed in: E.coli

HIST1H1C (Ab-62) Antibody
1-CSB-PA010378OA62ncrHU	Cusabio	EUR 332.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against HIST1H1C (Ab-62). Recognizes HIST1H1C (Ab-62) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:10-1:100

HIST1H1C (Ab-62) Antibody
1-CSB-PA010378OA62nsucHU	Cusabio	EUR 332.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against HIST1H1C (Ab-62). Recognizes HIST1H1C (Ab-62) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

HIST1H1C (Ab-62) Antibody
1-CSB-PA010378PA62nacHU	Cusabio	EUR 332.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against HIST1H1C (Ab-62). Recognizes HIST1H1C (Ab-62) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

HIST1H1E (Ab-62) Antibody
1-CSB-PA010380PA62nforHU	Cusabio	EUR 332.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against HIST1H1E (Ab-62). Recognizes HIST1H1E (Ab-62) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200

STMN1 (Ab-62) Antibody
CSB-PA082087-	Cusabio		EUR 335
Description: A polyclonal antibody against STMN1 (Ab-62). Recognizes STMN1 (Ab-62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200

STMN1 (Ab-62) Antibody
CSB-PA082087-100ul	Cusabio	100ul	EUR 316
Description: A polyclonal antibody against STMN1 (Ab-62). Recognizes STMN1 (Ab-62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200

BCL2L1 (Ab-62) Antibody
CSB-PA923588-	Cusabio		EUR 335
Description: A polyclonal antibody against BCL2L1 (Ab-62). Recognizes BCL2L1 (Ab-62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

BCL2L1 (Ab-62) Antibody
CSB-PA923588-100ul	Cusabio	100ul	EUR 316
Description: A polyclonal antibody against BCL2L1 (Ab-62). Recognizes BCL2L1 (Ab-62) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

stathmin1 (Ab-62) Antibody
21518-100ul	SAB	100ul	EUR 252

stathmin1 (Ab-62) Antibody
21518-50ul	SAB	50ul	EUR 187

ATF2 (pS44 / 62) Antibody
20-abx119178	Abbexa	EUR 314.00 EUR 98.00 EUR 398.00 EUR 495.00	100 ug 10 ug 200 ug 300 µg

Nucleoporin 62 (NUP62) Antibody
20-abx001943	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Nucleoporin 62 (NUP62) Antibody
20-abx142112	Abbexa	EUR 370.00 EUR 606.00 EUR 314.00	100 ul 200 ul 50 ul

Nucleoporin 62 (NUP62) Antibody
abx033433-400ul	Abbexa	400 ul	EUR 523

Nucleoporin 62 (NUP62) Antibody
abx033433-80l	Abbexa	80 µl	EUR 286

Nucleoporin 62 (NUP62) Antibody
abx433060-200ul	Abbexa	200 ul	EUR 286

Nucleopurin 62 kDa Protein
20-abx262076	Abbexa	EUR 4838.00 EUR 328.00 EUR 230.00	1 mg 20 ug 5 ug

Nucleoporin 62 (NUP62) Antibody
abx235927-100ug	Abbexa	100 ug	EUR 481

anti-stathmin1 (Ab-62)
LF-PA20689	Abfrontier	100 ul	EUR 334
Description: Rabbit polyclonal to stathmin1

KBA 62/Melanoma Associated Antigen
MO47032	Neuromics	100 ul	EUR 349

Recombinant HPV-62 L2 Protein
VAng-Wyb3365-inquire	Creative Biolabs	inquire	Ask for price
Description: Human papillomavirus type 62 Major capsid protein L1, recombinant protein.

BCL-XL (Ab-62) Antibody
21061-100ul	SAB	100ul	EUR 252

BCL-XL (Ab-62) Antibody
21061-50ul	SAB	50ul	EUR 187

Nucleoporin 62 kDa (NUP62) Antibody
20-abx128968	Abbexa	EUR 314.00 EUR 133.00 EUR 815.00 EUR 425.00 EUR 272.00	100 ug 10 ug 1 mg 200 ug 50 ug

Nucleoporin 62 kDa (NUP62) Antibody
20-abx114230	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Melanoma Marker(KBA.62) Antibody
BNC040895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF405S conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC040895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF405S conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC470895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF647 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC470895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF647 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNCAP0895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNCAP0895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNCB0895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), Biotin conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNCB0895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), Biotin conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC810895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF680R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC810895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF680R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC700895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF770 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC700895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF770 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC880895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF488A conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC880895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF488A conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC610895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF660R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC610895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF660R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC680895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF568 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC680895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF568 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNCA0895-250	Biotium	250uL	EUR 383
Description: Primary antibody against Melanoma Marker(KBA.62), APC conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNUM0895-50	Biotium	50uL	EUR 395
Description: Primary antibody against Melanoma Marker(KBA.62), 1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC050895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF405M conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC050895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF405M conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC400895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF640R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC400895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF640R conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNCP0895-250	Biotium	250uL	EUR 383
Description: Primary antibody against Melanoma Marker(KBA.62), PerCP conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNCR0895-250	Biotium	250uL	EUR 383
Description: Primary antibody against Melanoma Marker(KBA.62), RPE conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNUB0895-100	Biotium	100uL	EUR 209
Description: Primary antibody against Melanoma Marker(KBA.62), Concentration: 0.2mg/mL

Melanoma Marker(KBA.62) Antibody
BNUB0895-500	Biotium	500uL	EUR 458
Description: Primary antibody against Melanoma Marker(KBA.62), Concentration: 0.2mg/mL

Nucleoporin 62 kDa (NUP62) Antibody
20-abx242160	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Nucleoporin 62 kDa (NUP62) Antibody
20-abx242161	Abbexa	EUR 411.00 EUR 300.00	100 ul 50 ul

Nucleoporin 62 kDa (NUP62) Antibody
20-abx173878	Abbexa	EUR 857.00 EUR 439.00	1 mg 200 ug

Melanoma Marker(KBA.62) Antibody
BNC430895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF543 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC430895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF543 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNCH0895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNCH0895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC550895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF555 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC550895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF555 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC940895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF594 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC940895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF594 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC800895-100	Biotium	100uL	EUR 199
Description: Primary antibody against Melanoma Marker(KBA.62), CF680 conjugate, Concentration: 0.1mg/mL

Melanoma Marker(KBA.62) Antibody
BNC800895-500	Biotium	500uL	EUR 544
Description: Primary antibody against Melanoma Marker(KBA.62), CF680 conjugate, Concentration: 0.1mg/mL

stathmin1 (Ab-62) Conjugated Antibody
C21518	SAB	100ul	EUR 397

CART (62-76) (rat, human)
B5414-1	ApexBio	1 mg	EUR 383

CART (62-76) (human, rat)
H-5098.0001	Bachem	1.0mg	EUR 576
Description: Sum Formula: C64H99N17O23S3; CAS# [210978-19-1] net

CART (62-76) (human, rat)
H-5098.0005	Bachem	5.0mg	EUR 2207
Description: Sum Formula: C64H99N17O23S3; CAS# [210978-19-1] net

anti-BCL-XL (Ab-62)
LF-PA20050	Abfrontier	100 ul	EUR 334
Description: Rabbit polyclonal to BCL-XL