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Apo 3/7 HTS
High Throughput Screen for Caspase 3/7 detection
 
Key Benefits:
  • Homogenous assay for active caspase 3/7.
  • Breakthrough in cell lysis buffer and preservation of caspase activity.
  • Results in a No wash one-step assay
  • No need to wash out media from cell samples, just add the reagent directly to your experimental samples.
  • Easy to Use: No need to make cell lysates or run western blots.
  • Works with suspension and adherent cells.
  • Applications - Standard Fluorescence Plate Reader 
Assay Principle
Cell Technology’s Apo 3/7 HTS Assay utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The absorption and emission properties of the R110 dye are suppressed when attached to the z-DEVD peptide sequence. When R110 is cleaved away, by active caspase3/7, form the quenching DEVD sequence, the free dye excites at 488nm and emits at 515-530 nm.. As a result of a novel and proprietary Lysis Buffer System, the Apo 3/7 HTS Assay is a homogenous platform that can be utilized for high throughput fluorescence plate reader applications. The reagent is directly added to the samples thus eliminating any wash steps.
Kit contents
  • 1 vial Caspase 3/7 Reagent. (z-DEVD)2 Rhodamine 110 Part#4004
  • 1 Bottle of Cell Lysis Buffer
The following kits are available:
Catalog
 
Size
 
Price (Eur)
APO200-2
 
100 Tests €275
APO200-3 500 Tests
 
€745
APO200-4
 
1000 Tests
 
€1395