Home Up Call me back Contents Immunology Infectious agents Cellular Biology Molecular biology Instruments Chimical Product NEW antibodiesAche Peroxidation & Chlorination Detection Myeloperoxidase Detection - Eosinophil Peroxidase Detection Catalase Detection Monoamine oxidase A&B detection Semicarbazide sensitive amine oxidase detection Monoamine oxidase A&B detection

Google

       Pricelist 2010 

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

52074 Aachen, Germany

 Tel 0241 40 08 90 86

Fax 0241 55 91 05 36

GENTAUR Pol Sp. Z.o.o. Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 51 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

 

Fluoro MAO
Fluorescent Monoamine Oxidase A&B Detection Kit
 
Key Benefits:
  • Non Radioactive
  • Can monitor multiple time points to follow kinetics.
  • One-step, no wash assay.
  • Adaptable for High Throughput format
  • Sensitive
  • Applications - Standard Fluorescence Plate Reader
Assay Principle
Monoamine oxidase (MAO) is a flavin-containing enzyme that catalyses the oxidation of a variety of amine-containing neurotransmitters such as serotonin, norepinephrine, epinephrine and dopamine to yield the corresponding aldehydes (1). MAO exists in two isoforms, namely MAO-A and MAO-B, which are the products of two distinct genes (2).

MAO-A and B exhibit different specificities to substrates and inhibitor selectivities. Extensive studies have been preformed to characterize their properties (3-7). MAO-A acts preferentially on serotonin and norepinephrine, and is inhibited by clorgyline. MAO-B acts preferentially on 2-phenylethylamine and benzylamine and is inhibited by deprenyl and pargyline.

Localized in the outer mitochondrial membrane, these enzymes are found throughout the body. Often only one form of the enzyme is present in a specific organ and/or within a specific cell type (8-9). In addition to their role in regulating neurotransmitters, these enzymes are also involved in processing biogenic amines (10) including tyramine (11).

The Fluoro MAO-A/B detection kit utilizes a non - fluorescent substrate, 10-Acetyl-3, 7-dihydroxyphenoxazine (ADHP) to detect H202 released from the conversion of a substrate to its aldehyde via MAO-A/B. Furthermore, H202 oxidizes ADHP in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase.

Reaction:
Substrate + O2 + H202 + MAO-A/B ----------> aldehyde + NH3 + H202

H202 + Detection reagent (non-fluorescent) + Peroxidase ---------> Resorufin (fluorescent)

The fluorescent monoamine oxidase detection kit can be used to monitor MAO activity and screen of MAO inhibitors.
Kit contents (for 500 assays)
Description
 
Part#
 
Storage after opening Kit or Reconstitution of Reagents.
Bottle: 5X Reaction Buffer pH 7.4
 
3020 4-80C
1 vial: Detection reagent 4009
 
Aliquot in single use vials: Below –200C
1 vial: Horseradish Peroxidase 6007 4-80C
1 vial: MAO-B substrate Benzylamine
 
7006 Aliquot in vials: Below –200C
1 vial: MAO-A/B substrate: Tyramine 7005 Aliquot in single use vials: Below –200C
1 vial Pargyline: Monoamine Oxidase B inhibitor (12-14) 7003 Aliquot in vials: Below –200C
1 vial Clorgyline: Monoamine Oxidase A inhibitor(12-14) 7002 Aliquot in single use vials: Below –200C
The following kits are available:
Catalog #
 
Size
 
Price (US$)
FLMAO 100-3
 
500 Tests $245