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Fluoro MPO™
Fluorescent Myeloperoxidase Detection Kit
 
Key Benefits:
  • Can monitor multiple time points to follow kinetics.
  • One-step, no wash assay.
  • Adaptable for High Throughput format
  • Highly Sensitive
  • Applications - Fluorescence (Plate Reader) or Absorbance
Assay Principle
Myeloperoxidase (MPO) is a highly cationic glycosolated hemoprotein that has a molecular weight of 144kD. The hemoprotein consists of two dimers linked via a disulfide bridge. Each dimmer is composed of a heavy (53kD) and light (15kD) subunit. Each heavy chain contains an independently acting protoporphyrin group containing a central iron (1-5). MPO is present in the azurophilic granules of polymorphonuclear leukocytes (PMNs) and is unique to neutrophils and monocytes. However, monocytes contain only one third of the MPO found in PMN’s. MPO utilizes H202 produced by the neutrophils to oxidize a varity of aromatic compounds to give substrate radicals for bactericidal activity (4 review). This enzyme is unique however in that it can oxidize chloride ions to produce a strong nonradical oxidant,HOCl. HOCl is the most powerfull bactericidal produced by neutrophils (4 review). Excessive production of these radicals can cause oxidative stress leading to oxidative tissue injury.  
H2O2 + Detection reagent (non-fluorescent)+ MPO
                                             ---------> fluorescent analog 
                                     Excitation:530-571nm; Emission: 590-600nm
 
Applications
  • Detection of MPO activity in neutrophils and macrophages.
  • Detection of PMN infiltration in tissue samples (inflammation and innate host defense mechanisms).  
  • Acute and chronic inflammatory disorders due to oxidative tissue damage.  
  • MPO activity in acute and chronic manifestations of cardiovascular disease.
References
The following kits are available:
Catalog 
 
Size
 
Price (Eur)
FLMPO 100-3
 
500 Tests €395