|The Fluoro NAD/NADH
detection kit utilizes a non-fluorescent detection
reagent, which is oxidized in the presence NADH to produce its
fluorescent analog and NAD. NAD is further converted to NADH via
an enzyme coupled reaction. The enzyme reaction specifically
reacts with NAD/NADH and not with NADP/NADPH.
1. NADH +
non-fluorescent detection reagent+ electron coupler ----->
analog + NAD
2. NAD + enzyme coupled
reaction -------> NADH (then proceeds to reaction 1).
excitation: 530-570nm and
emission at 590-600nm