Cell Technology’s Mito Flow assay utilizes a cationic dye to
visualize mitochondrial membrane potential (5-7). The Mito
Flow reagent is a cell permeable cationic dye that has a
strong fluorescent signal and exhibits low membrane
potential independent (non specific) binding and toxicity.
In healthy cells the Mito Flow reagent is accumulated by the
mitochondria in proportion to the DeltaPsi (membrane
potential). In most cell lines, accumulation of the Mito
Flow reagent in the mitochondria results in a higher
fluorescence intensity. In apoptotic cells, where the
mitochondrial membrane potential is compromised, the Mito
Flow reagent does not get accumulated in the mitochondria
and these cells exhibit a lower fluorescence signal.