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Fluoro Catalase
Fluorescent Catalase Detection Kit
Key Benefits:
  • Sensitive Fluorescent Assay
  • Enzyme Positive Control included in kit.
  • Can monitor multiple time points to follow kinetics.
  • One-step, No Wash assay.
  • Adaptable for High Throughput format.
  • Monitors enzymatic activity.
  • Applications - Any standard Fluorescent Plate Reader.
Assay Principle
Catalase is an antioxidant enzyme that catalyses the decomposition of hydrogen peroxide (H202) to water and oxygen. Catalase is ubiquitously expressed in mammalian and non-mammalian aerobic cells containing the cytochrome system. The enzyme has been isolated from various sources, including bacteria and plant cells (1-3). Catalase activity varies greatly from tissue to tissue. Highest activity is seen in liver and kidney, while lowest activity is seen in connective tissue (3). In eukarotic cells, catalase in concentrated in organelles called peroxisomes (4).

The production of hydrogen peroxide in eukaryotic cells is an end product result of various oxidases and superoxide dismutase reactions. Accumulation of H202 can result in cellular damage through oxidation of proteins, DNA and lipids thus resulting in cell death and mutagenisis (8-11). H202 role in oxidative stress related diseases have been widely studied (8,12).

The Fluoro Catalase detection kit is sensitive assay that utilizes a non - fluorescent detection reagent to detect H202 substrate left over from the catalase reaction (5-6).

Reaction:
H202 + catalase ----------> Water + oxygen

H202 (Left Over) + Detection reagent (non-fluorescent) + Peroxidase ---------> Resorufin (fluorescent)

Excitation 530-571
Emission 590-600nm

Kit contents (for 500 assays)
Description
 
Part
 
Storage after opening Kit or
Reconstitution of Reagents.
1 Bottle: 5X Reaction Buffer pH 7.4
 
3021 4-80C
1 vial: Detection reagent 4010
 
Undiluted : 4-80C

Diluted: Aliquot in single use vials: Below –200C

1 vial: Horseradish Peroxidase 6009 4-80C undiluted. Diluted: below –200C.
1 vial: Hydrogen Peroxide 3022 4-80C.
1 vial: Catalase enzyme 6008 Crystalline suspension: 4-80C
The following kits are available:
Catalog 
 
Size
 
Price (Eur)
FLOCAT 100-3
 
500 Tests 245 €