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Protéin A

Product Name

Recombinant A 

Price

150 € / 500ul

Source

Sequence                     

E coli

 Amino acid 32 -327 of the Staphylococcus aureus subsp aureus . Protein A lg binding domains with 6X Hist-tag on N-terminus . Gene Bank Accession Number YP_498670  

   

Purity

>99 % by SDS – PAGE and HPLC analyses . Endotexin level is < 0.1 EU /mg of Protein A

Mol Weight

39.2 Kda

Form

Lyophilized

Raised in

Rabbit

Clonality

Polyclonal

Form

Crude serum

Storage instructions

Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Expiration

20/04/2016

 

Application :

The recombinant Protein A is a genetically engineerd protein containing five lg- binding regions of protein A and 6X Hist – tag on N terminus . Cell wall binding region and other non specific binding region and other non specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific  lgG binding . The 6 X His- tag on N terminus can be used for affinity purification  or for protein A detection using  anti Hist- tag antibody .

The  recombinant  Protein A is deal for purification of polyclonal or monoclonal  lgG antibodies .

 

Protein A binds to most human and mouse lgG subclasses ( eg human , lgG1 , lgG2, lgG4; mouse lgG1, lgG2a, lgG2b, lgG3 ) It also binds to total lgG from cow , guinea pig

 

Usage : For Research Purpose Only . Not to be used in Human