Home Up Call me back Contents Immunology Infectious agents Cellular Biology Molecular biology Instruments Chimical Product NEW antibodiesTris-Glycine-SDS PAGE Buffer (10X) ProtoGel Resolving Buffer (4X) ProtoGel Stacking Buffer Tris-Tricine-SDS PAGE Buffer (10X) Tris-Glycine Electroblotting Buffer (10X) PBS (10X) Phosphate Buffered Saline Tris-Tricine-SDS PAGE Buffer (10X)

Google

       Pricelist 2010 

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

52074 Aachen, Germany

 Tel 0241 40 08 90 86

Fax 0241 55 91 05 36

GENTAUR Pol Sp. Z.o.o. Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 51 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

 

Tris-Tricine-SDS PAGE Buffer (10X)

 
 
  • Running Buffer for Small Proteins
  • Variation on the Schagger/von Jagow System
  • Substitute for Tris-Glycine-SDS in the Standard Laemmli Protocol
Discontinuous SDS-PAGE employing Tris-Glycine as the tank buffer resolves proteins down to about 15 kd. However, below this size, the proteins do not destack from the SDS micelles running through the gel with the buffer front. In order to resolve proteins in this size range, the Tris-Tricine system of Schagger and von Jagow (1987) was developed.
An Alternative to the Schagger and von Jagow System involves running Tris-Tricine Gels using National Diagnostics 10X Tris-Tricine-SDS Buffer (EC-869). With National Diagnostics Tris-Tricine-SDS, you can extend the range of SDS-PAGE to resolve smaller proteins with minimal alteration of protocol.
To provide this level of convenience, National Diagnostics streamlined the original method of Shaggar and Von Jagow (1987 Anal. Biochem 166) by developing Tris-Tricine-SDS cathode tank buffer to be compatible with the standard Laemmli gel/buffer system. This combination resolves proteins as small as 5kD. The researcher simply substitutes National Diagnostics Tris-Tricine-SDS in the upper (cathode) tank, with no other changes from standard Laemmli protocol to extend the resolution of their gels.

Catalog Number: EC-869
 

APPLICATIONS OF TRIS-TRICINE-SDS PAGE BUFFER (10X)
Electrophoresis Buffers
Denaturing Protein Electrophoresis: SDS-PAGE
Related Products   
ProtoGel Quick-Cast 12%
Ready-to-use gel solution for SDS-PAGE that combines the convenience of pre-cast gels with the economy of casting your own.
 
ProtoGel (30%)
30% concentrated solution of acrylamide and bis-acrylamide, 37.5 : 1 ratio. Filtered, deionized, and stabilized.
 
ProtoGel (40%)
Concentrated solution of acrylamide and bis-acrylamide, 37.5 : 1 ratio. Filtered, deionized, and stabilized.
 
AccuGel 29:1
Concentrated solution of acrylamide and bis-acrylamide (29:1) for native DNA electrophoresis and SDS-PAGE. Filtered, deionized, and stabilized.
 
Tris-Glycine-SDS PAGE Buffer (10X)
Ultra-pure concentrated standard running buffer for SDS-PAGE. Formulated with 18 MegOhm water. 0.2 micron filtration.
 
ProtoGel Resolving Buffer (4X)
4X Laemmli Tris-HCl resolving gel buffer for clear, reproducible SDS-PAGE.
 
ProtoGel Stacking Buffer
4X Laemmli Tris-HCl stacking gel buffer for clear, reproducible SDS-PAGE.
 
Protein Loading Buffer Blue (2X)
Ready to use sample buffer for SDS-PAGE.
 
Protein Loading Buffer Blue (2X)
Ready to use sample buffer for SDS-PAGE.
 
ProtoBlue Safe
Eco-friendly, ultra-sensitive colloidal coomassie G-250 stain.
 
ProtoMarkers
Broad range pre-stained protein markers for SDS-PAGE.
 
Ammonium Persulfate - ULTRA PURE
Exceeds ACS standards. Low absorbed water results in consistent initiation.
 
TEMED - ULTRA PURE
Fractionally distilled to remove all trace metals and amine impurities. Stored under nitrogen.