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Introducing Ziva™

Ultrasensitive BrdU Cell Proliferation Assay

Research Use Only

Potential Uses

Cancer

- Detection of proliferating tumor cells or metastatic cells in blood

- Detection of tumor-specific T-cells

- Detection of stimulatory or inhibitory/toxic effects of various compounds/drug candidates on tumor or normal, primary or cell line viability

Immunology

- Detection of immunity using small numbers of cells

- Early detection of cell proliferation

- Detection of antigen specific T-cell proliferation

- Detection and quantification of the immunoreactivity of lymphocytes, stimulatory mitogens or antigens, after exposure to various cytokines or growth factors. Antigen specificity.

Autoimmunity/Transplantation

- Detection of autoreactive T-cells

- Comparison of responses of donor vs host samples

Recombinant Expression Technology

- Detection of transfected cells

Stem Cells

- Quality assessment of stem cells under various media conditions

The Ziva Performance Difference

- 200 x more sensitive than 3H-thymidine (the "Gold Standard")

- A broader dynamic range than 3H-thymidine

- A lower limit of detection: 1-4 proliferating cells in a background of 100,000 viable non-proliferating cells

- Simple to use, non-isotopic, 96 microtiter plate, ELISA format

- Assay performance time: < 1 hour

- Flexibility in small sample size (1 to 105 cells) and sample volume (50 μL to 100 μL), are ideal for longitudinal in vivo studies in rodents.