Filter-Based Protein Digestion (FPD): A Detergent-Free and Scaffold-Based Strategy for TMT Workflows.
High-throughput proteome profiling requires thorough optimization to attain complete evaluation. We developed a filter aided pattern preparation (FASP)-like, detergent-free methodology, termed Filter-Based Protein Digestion (FPD). We in contrast FPD to protein extraction strategies generally utilized in isobaric tag-based proteome profiling, particularly trichloroacetic acid (TCA) and chloroform-methanol (C-M) precipitation. We divided a mammalian complete cell lysate from the SH-SY5Y neuroblastoma
